Improved detection of methylation in ancient DNA
More On Article
- HEAS Member Discusses Tischoferhöhle on Austrian National Television
- HEAS Seed Grants June 2025 Round
- Decoding genomic landscapes of introgression.
- New insights from the application of ZooMS to Late Pleistocene fauna from Grotta di Castelcivita, southern Italy
- Exploring Ancient Foodways - An archaeological journey into Roman dietary habits at Kinderuni Wien 2025
Sawyer, S., Gelabert, P., Yakir, B., Llanos-Lizcano, A., Sperduti, A., Bondioli, L., Cheronet, O., Neugebauer-Maresch, C., Teschler-Nicola, M., Novak, M., Pap, I., Szikossy, I., Hajdu, T., Moiseyev, V., Gromov, A., Zariņa, G., Meshorer, E., Carmel, L., Pinhasi, R., 2024. Improved detection of methylation in ancient DNA. Genome Biology 25, 261.
Abstract
Reconstructing premortem DNA methylation levels in ancient DNA has led to breakthrough studies such as the prediction of anatomical features of the Denisovan. These studies rely on computationally inferring methylation levels from damage signals in naturally deaminated cytosines, which requires expensive high-coverage genomes. Here, we test two methods for direct methylation measurement developed for modern DNA based on either bisulfite or enzymatic methylation treatments. Bisulfite treatment shows the least reduction in DNA yields as well as the least biases during methylation conversion, demonstrating that this method can be successfully applied to ancient DNA.